Izinto ezisisiseko zokumisa izityalo (lyophilization)
I-lyophilization, eyaziwa nangokuthi i-freeze-drying, yinkqubo esetyenziselwa ukulondoloza izinto eziphilayo ngokususa amanzi kwisampuli, okubandakanya kuqala ukukrazula isampula bese uyayomisa, phantsi kwe-vacuum, kwiqondo eliphantsi kakhulu. Iimodeli eziphilileyo zigcinwa ixesha elide kunezampulu ezingaphendulwanga.
Kutheni Kusetyenziswa I-Lyophilization
Ukuphilisa i-Lyophilization, okanye ukumisa ama-bacterial cultures , ukuzinzisa iinkcubeko ngokugcina ixesha elide ngexesha lokunciphisa umonakalo onokubangelwa ngokumisa isampuli.
Uninzi lwezilwanyana ezincinci luphila kakuhle xa lyophilized kwaye lukwazi ukuvuselelwa lula kwaye lukhulile kwimithombo yeendaba, emva kwexesha elide lokugcina.
I-lyophilization isetyenziselwa kwi- biotechnology nakwiimveliso zendalo ukuze kugcinwe iigciwane, iisampuli zegazi, iiprotheni ezihlambulukileyo kunye nezinye izinto eziphilayo.
Le nkqubo encinci ye-laboratory ingasetyenziselwa nayiphi na isomisi yomdaka okhoyo ngokurhwebayo ukuze ulondoloze ukuqokelela kwenkcubeko yakho.
Inkqubo yokuLyophilization
Inkqubo yokwenza i-lyophilization ngokwenene iyisistim sezinto ezibonakalayo ezibizwa ngokuba yi-sublimation: ukuguqulwa kwezinto ezisuka kwisigxina ukuya kummandla we-gaseous, ngaphandle kokuqala ukudlula kwisigaba samanzi. Ngethuba le-lyophilization, amanzi kwisampuli echwayitiweyo asuswa njengomphunga wamanzi, ngaphandle kokuba athathe isampuli kuqala.
Iimpazamo eziqhelekileyo
Enye yeziphene eziqhelekileyo xa kuziwa kwi-lyophilization ayikwazi ukuba isampuli yakho idibeneyo, eyenza kunzima ukukhetha i-lyophilizer echanekileyo.
Iisampuli zakho zingancibilika ngexesha leenkqubo. Enye impazamo eqhelekileyo icinga ukuba i-colder ingcono xa i-frozen-drying kwi-shelf-uhlobo olumileyo olomileyo. Ngethuba lokumisa okuphambili, kufuneka usethe ukushisa kwetrhefri nje ngezantsi kweesampuli ze-eutectic lokushisa. Kufuneka kube nokushisa okwaneleyo ukukhuthaza iimlekyuli zeesampuli ukuba zihambe-kodwa zikhusele ukutyungulwa.
Impazamo yesithathu isebenzisa izixhobo ezingafanelekanga kwiisampuli zakho. Ngenxa yokuba i-dryer dryer isetyenziswe kwisilungiselelo seqela, kufuneka uwazi oku okulandelayo ngaphambi kokuthenga enye:
- Ubungakanani obumanyanisiweyo obuya kuthathwa lyophilized
- Yiyiphi isampuli (kunye nobushushu be-eutectic)
- Indlela yokusebenzisa kakuhle isomisi yomngcipheko
Ukuba leyunithi ayisebenzisi kakuhle, inokutshabalalisa zonke iisampuli. Oko kusilethela kwenye impazamo eqhelekileyo: Ukungagcini ipompo yokucima. Umpompo kufuneka ube ngowona myalelo osebenzayo wokusebenza kwe-lyophilization ukusebenza. Ukuqhuma umpompo kunye ne-ballast yegesi evulekileyo imizuzu engama-30 ngaphambi nangemva kokuba inkqubo yomisa eyomileyo iyakwandisa ubomi bompompo. Ukuvula i-ballast yegesi ihlambulula ukungcola kwipompo ukukhusela umonakalo kumacandelo angaphakathi.Ufanele ukhangele i-pump yeoli rhoqo ukulungelelanisa kunye neengqungquthela, kwaye utshintshe ioli njengoko kuyimfuneko. Ukutshintsha kweoli rhoqo kugcina umpu odonsa kwi-vacuum ephezulu ngexesha lomsebenzi omile.
Ekugqibeleni, ukuba neefowuni ezingenambi zokumisa kwinkqubo yakho yokwenza i-lyophilization ingaba yimpazamo enkulu. Ngaba udinga isampula yokuyeka phantsi kwe-vacuum? Emva koko kufuneka igumbi lokumisa. Ngaba unqabile-omisa kwiiflasks? Emva koko qiniseka ukuba ube nekamelo lokumisa kunye namachweba.
Ngokuziphepha iimpazamo ezingentla, unokwenza unonophelo olumgangatho wokumisa umnqamlezo wakho kunye nompompo, kwaye ube neesampuli ezingcono xa ukomiswa kwakho komngcingo kwenziwa.
Iingxelo
Iindaba zeLabconco. "Iiphosiso eziphezulu ezi-5 zenziwe kwinkqubo yokulungisa i-lyophilization." http://www.labconco.com/news/top-5-lyophilization-mistakes