I-sodium citrate buffer isetyenziselwa ukuhlukaniswa kwe-RNA ngenxa yokunciphisa i-hydrolysis yesiseko se-RNA. Oku kubangela inyathelo elibalulekileyo ekuhlambulukeni kwe-MRNA ngexesha lophando lwe-genomic okanye ukufundela umbhalo.
Izikhungululi ezisemgangathweni zinganceda ukufumanisa ama-antigens kwizilungiso eziphathekayo zamathambo ngenxa yokuba ziphule izikhonkwane eziphambili phakathi kwama-antigens kunye neendaba zokulungisa.
Ngesikhokelo esingezantsi, yenza i-sodium citrate buffer ye-pH 6. Imiyalelo ayilula nje kuphela kodwa ikhawuleza ukuphumeza kwakhona. Kumele kukuthathele malunga nemizuzu eyi-10 ukugqiba eli nyathelo.
Izinketho ezimbini zokwenza iBamp
Kukho iindlela ezimbini zokuhamba malunga nokwenza i-sodium citrate buffer. Okokuqala, qaphela ukuba unayo i-citric acid kunye ne-conjugate base, i-citrate ye-sodium, ukusebenzisana nayo. Ukuba unayo i-citric acid kuphela, akuyona ingxaki. Unokwenza i-buffer.
Ngakolunye uhlangothi, ukuba unayo i-asidi kunye nesiseko, yenza isisombululo sesithamo ngasinye. Hlanganisa ama-gramu angama-21 e-citric acid kwi-1 litre yamanzi adibeneyo, kunye ne-29.4 gram ye-sodium citrate kwi-1 litre yamanzi adibeneyo.
Ukuba unayo i-citric acid kuphela, xuba 2.1 grams kwi-1 ilitha engama-distilled water uze uqhubeke nesinyathelo esilandelayo.
Ukudibanisa i-Citric Acid kunye neSicombate Citrate Solutions
Ngoku ukuba udale isisombululo sakho se-stock (ukuba unayo i-asidi kunye nesiseko) okanye udibanise i-asidi yakho kumanzi adibeneyo (ukuba awuzange), unokugxila kwizisombululo zakho.
Hlanganisa i-82 milliliters yesisombululo se-citric acid kunye ne-18 milliliters yesisombululo se-sodium citrate.
Emva koko, yenza uwonke umthamo womxube ukuya kwi-litre engama-1 kunye namanzi ane-distilled. Shiya umthamo okwaneleyo ukuvumela ukongezwa kwe-sodium hydroxide (NaOH) kwisinyathelo esilandelayo.
Ukulungisa i-pH
Ngoxa ulungelelanisa ngesisombululo ngokusebenzisa i-magnetic stirrer, sebenzisa i-1M ye-sodium hydroxide ukulungisa i-pH yomxube ukuya ku-6.0. Emva koko, yenza umthamo wokugqibela wesisombululo ukuya kwi-1 litre ngamanzi adibeneyo ngokusebenzisa i-flaum volumetric.
Oko Kufuneka Ukwenza I-Buffer
Akunakukuthatha nje ixesha elide ukuba wenze i-sodium citrate buffer, nawe akayi kufuna iimpahla ezininzi. I-citric acid, i-1M ye-sodium hydroxide, amanzi adibeneyo, kunye neprojelo ye-pH elandeleleneyo, uya kuba sekunye apho. Ukusebenzisa i-citrate ye-sodium ikhetho.
Ukwenza i-buffer iya kufuna i-cylinder e-1 ilitha ephunyeziweyo, i-1-litre ye-volumetric flask, kunye ne-1 litre yeebhodlela zemidiya. Ekugqibeleni, uya kufuna umbhobho wokugubha umbane kunye nomqhubi wamagnetic.
Ukuba awunayo yonke le mveliso, cinga ukusebenzisa i-lab kwisikolo sakho okanye kwindawo yomsebenzi, ngoko akufanele uzithenge. Ungakwazi ukuthenga lula izinto eziphathekayo kwi-intanethi okanye ukutyelela ivenkile yezinto ezikhethekileyo kwindawo yakho.